Figure 1 – Plates of E. Coli BL21 (DE3). The control is on the right (E. Coli present by no DNA present). The experimental plate is in the middle (E. Coli present with pGEM-gbr22 (plasmid C)). The fun plate is on the left, which has bacteria from the computer keyboards.
Figure 2 – Flask of purple culture. E. Coli BL21 (DE3) was transformed with pGEM-gbr22 (plasmid C)).
Figure 3 – Tube of purple cell pellet. E. Coli BL21 (DE3) was transformed with pGEM-gbr22 (plasmid C)).
Figure 4 – Tube of Elution 1 solution. 4.8 mL pGEM-gbr22 (plasmid C) was released from the Ni-NTA resin after 5 mL of elution buffer (1x PBS and 250 mM imidazole) was added.
Figure 5 – Tube of Elution 2 solution. 5.0 mL pGEM-gbr22 (plasmid C) was released from the Ni-NTA resin after 5 mL of elution buffer (1x PBS and 250 mM imidazole) was added after obtaining Elution 1 solution.
Figure 6a – First trial for absorbance of 0.134 at 280 nm wavelength from 2 ul of Elution 1 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 6b – Second trial for absorbance of 0.137 at 280 nm wavelength from 2 ul of Elution 1 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 7a – First trial for Absorbance of 0.33 at 574 nm max wavelength from 2 ul of Elution 1 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 7b – Second trial for Absorbance of 0.29 at 574 nm max wavelength from 2 ul of Elution 1 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 8a – First trial for absorbance of 0.005 at 280 nm wavelength from 2 ul of Elution 2 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 8b – Second trial for absorbance of 0.004 at 280 nm wavelength from 2 ul of Elution 2 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole)
Figure 9a – First trial for absorbance of 0.006 at 574 nm wavelength from 2 ul of Elution 2 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 9b – Second trial for absorbance of 0.007 at 574 nm wavelength from 2 ul of Elution 2 (pGEM-gbr22 (plasmid C)) after initializing with 2 ul of nanopure water and blanking with 2 ul of elution buffer (1X PBS and 250 mM imidazole).
Figure 10 - Figure 1 - Gel after staining. Sample was rinsed with Nanopure water and KimWipe was used to soak up excess stain. Gel was placed on Whatman paper after being left overnight on the orbital shaker. The first well was the ladder, the second well was sample 1, the third well was sample 2, the fourth well was skipped to due poor pipetting, the fifth well was sample 3, the sixth well was skipped due to poor pipetting, the seventh well was sample 4, the eighth well was sample 5, the ninth well was sample 6, and the tenth well was my partner's sample 5.
Figure 11 - Gel after 1.5 hours of drying at 75 degrees celsius. Sample was placed on Whatman paper and covered with cellophane. The first well was the ladder, the second well was sample 1, the third well was sample 2, the fourth well was skipped to due poor pipetting, the fifth well was sample 3, the sixth well was skipped due to poor pipetting, the seventh well was sample 4, the eighth well was sample 5, the ninth well was sample 6, and the tenth well was my partner's sample 5.
Title:
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References: